tuaA/2

lipid carrier sugar transferase, disrupted pseudogene in B. subtilis 168

locus: BSU_35610

pI: 0.00

mw: 0.00 kDa

protein length: 162 aa

gene length: 486 bp

function: biosynthesis of teichuronic acid

product: lipid carrier sugar transferase

essential: no

synonyms: yvhA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.5|Biosynthesis of teichuronic acid]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.4|Biosynthesis of teichuronic acid]
• [category|SW 6|Groups of genes] → [category|SW 6.10|Pseudogenes]

Gene

Coordinates: 3,658,407 → 3,658,892

The protein

Catalyzed reaction/ biological activity

• di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-α-D-galactosamine --> N-acetyl-α-D-galactosaminyl-1-diphospho-di-trans,octa-cis-undecaprenol + UMP (according to UniProt)

Protein family

• Bacterial sugar transferase family (with [protein|D67665412B78B56752B460219445E4BEF52C3A1A|EpsL], according to UniProt)

Paralogous protein(s)

• [protein|D67665412B78B56752B460219445E4BEF52C3A1A|EpsL]

Structure

• [PDB|5W7L] (from Campylobacter concisus, 41% identity)

Expression and Regulation

Operons

• genes: [gene|5790E3DB39F31017B557C2D3F428C0348A461DA6|tuaA/2]-[gene|4AC72C556ED02A7DE4E1E8D69A0FC926359ACE28|tuaA/1]-[gene|54F96DE549F78E6C1580949A21C84B27AF26C4C6|tuaB]-[gene|873EE7A8C9BBFC6E96B0957B450BA665E3C9222D|tuaC]-[gene|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|tuaD]-[gene|B89FA71C2924CC0690224AFBFA06DB0FF0D97C0A|tuaE]-[gene|6F0FA23892BB1829174F66C464149DB428A8DE18|tuaF]-[gene|6E4F2BA67FF58085D462F6A6FB6497BDF600FCF6|tuaG]-[gene|737B37DA0DA1642AD5C4A824228BFED55FF0652A|tuaH]
  description: [Pubmed|10048024]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10048024], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325,15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|9611818,10627039], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

  regulation

  • expressed during [SW|sporulation] in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|16497325,15699190]

Biological materials

Mutant

• BKE35610 (Δ[gene|5790E3DB39F31017B557C2D3F428C0348A461DA6|tuaA/2]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGACACACACCTCACGA, downstream forward: _UP4_GAGATTCCAGGCTTTACACA
• BKK35610 (Δ[gene|5790E3DB39F31017B557C2D3F428C0348A461DA6|tuaA/2]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGACACACACCTCACGA, downstream forward: _UP4_GAGATTCCAGGCTTTACACA

References

• 9683503,9611818,10627039,10048024