ybgG

homocysteine methyltransferase

locus: BSU_02410

pI: 4.86

mw: 34.58 kDa

protein length: 315 aa

gene length: 948 bp

function: biosynthesis of methionine

product: homocysteine methyltransferase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.11|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine/ based on similarity]

Gene

Coordinates: 261,656 262,603

The protein

Catalyzed reaction/ biological activity

• S-methyl-L-methionine + L-homocysteine --> 2 L-methionine (according to UniProt)

[SW|Domains]

• Hcy-binding domain (aa 2-309) (according to UniProt)

[SW|Cofactors]

• Zn²⁺ (according to UniProt)

Structure

• [PDB|5DML] (from ''Escherichia coli'', 52% identity) [pubmed|26564203]

Expression and Regulation

Operons

• genes: [gene|577723B2BF662A44D55DC84444ED64276D8DED17|ybgG]
  description: [Pubmed|15995196]

• genes: [gene|577723B2BF662A44D55DC84444ED64276D8DED17|ybgG]-[gene|B6A920650919D5BBBD75CCA58C30A0D8ECE64DA3|ybgF]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B941 (ybgG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1940 NBRP B. subtilis, Japan]
• BKE02410 ([gene|577723B2BF662A44D55DC84444ED64276D8DED17|ybgG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGAATGTACCGATGCCT, downstream forward: _UP4_TGACTTATCGGAATAGTTTG
• BKK02410 ([gene|577723B2BF662A44D55DC84444ED64276D8DED17|ybgG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGAATGTACCGATGCCT, downstream forward: _UP4_TGACTTATCGGAATAGTTTG

References

• 11267663,15995196,9882684,11898408,26564203