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cheV
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cheV

modulation of CheA activity in response to attractants
Locus
BSU_14010
Isoelectric point
4.62
Molecular weight
34.48 kDa
Protein length
303 aa Sequence Blast
Gene length
912 bp Sequence Blast
Function
control of CheA activity
Product
CheA modulator
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,473,605 1,474,516

Phenotypes of a mutant

  • cheV cheW double mutants exhibit complete loss of chemotaxis PubMed
  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
  • The protein

    Paralogous protein(s)

    Domains

  • N-terminal CheW-like domain, C-terminal two-component receiver domain PubMed
  • Response regulatory domain (aa 176-302) (according to UniProt)
  • CheW-like domain (aa 16-154) (according to UniProt)
  • Modification

  • the C-terminal two-component receiver domain is phosphorylated on a Asp residue by CheA PubMed
  • Localization

  • forms lateral clusters (phosphorylated form), but in the presence of high asparagine concentration (non-phosphorylated form) there is a reversible re-localization to the poles of the cell PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon
  • Additional information

  • in minimal medium, CheV is present with 7,500 /- 2,000 molecules per cell PubMed
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    Biological materials

    Mutant

  • DS70 (cheV::mls in NCIB3610) PubMed
  • BKE14010 (cheV::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTCAATCCCTCGCTATT, downstream forward: _UP4_TAAATAAAAACAGCCGTTGC
  • BKK14010 (cheV::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTCAATCCCTCGCTATT, downstream forward: _UP4_TAAATAAAAACAGCCGTTGC
  • References

    Reviews

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    Original publications

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