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cheV
modulation of CheA activity in response to attractants
Molecular weight
34.48 kDa
Function
control of CheA activity
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
1,473,605 1,474,516
Phenotypes of a mutant
cheV cheW double mutants exhibit complete loss of chemotaxis PubMednot essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed The protein
Paralogous protein(s)
Domains
N-terminal CheW-like domain, C-terminal two-component receiver domain PubMedResponse regulatory domain (aa 176-302) (according to UniProt)CheW-like domain (aa 16-154) (according to UniProt) Modification
the C-terminal two-component receiver domain is phosphorylated on a Asp residue by CheA PubMed Localization
forms lateral clusters (phosphorylated form), but in the presence of high asparagine concentration (non-phosphorylated form) there is a reversible re-localization to the poles of the cell PubMed Expression and Regulation
Operons
Sigma factors
Additional information
in minimal medium, CheV is present with 7,500 /- 2,000 molecules per cell PubMed view in new tabBiological materials
Mutant
DS70 (cheV::mls in NCIB3610) PubMedBKE14010 (cheV::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTCAATCCCTCGCTATT, downstream forward: _UP4_TAAATAAAAACAGCCGTTGCBKK14010 (cheV::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTCAATCCCTCGCTATT, downstream forward: _UP4_TAAATAAAAACAGCCGTTGC References
Reviews
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Original publications
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