cheV

modulation of CheA activity in response to attractants

locus: BSU_14010

pI: 4.62

mw: 34.48 kDa

protein length: 303 aa

gene length: 912 bp

function: control of CheA activity

product: CheA modulator

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.3|Control of response regulators/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.2|Coupling proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 1,473,605 1,474,516

Phenotypes of a mutant

• ''[gene|56E391BD4C154A603FFD06CEC7BC5615AAC5AD04|cheV] [gene|887D84520DF3D5F22DED525C1C17130EDE60DC36|cheW]'' double mutants exhibit complete loss of chemotaxis [Pubmed|21098025]
• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]

The protein

Paralogous protein(s)

• [protein|887D84520DF3D5F22DED525C1C17130EDE60DC36|CheW]:

[SW|Domains]

• N-terminal [protein|887D84520DF3D5F22DED525C1C17130EDE60DC36|CheW]-like domain, C-terminal two-component receiver domain [Pubmed|11553614]
• [SW|Response regulatory domain] (aa 176-302) (according to UniProt)
• CheW-like domain (aa 16-154) (according to UniProt)

Modification

• the C-terminal two-component receiver domain is phosphorylated on a Asp residue by [protein|6B3B222E56BF0C95A2371CA5208B5522B44D4689|CheA] [Pubmed|11553614]

[SW|Localization]

• forms lateral clusters (phosphorylated form), but in the presence of high asparagine concentration (non-phosphorylated form) there is a reversible re-localization to the poles of the cell [Pubmed|21098025]

Expression and Regulation

Operons

• genes: [gene|56E391BD4C154A603FFD06CEC7BC5615AAC5AD04|cheV]
  description: [Pubmed|8169223]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|8169223], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  additional information

  • in minimal medium, CheV is present with 7,500 /- 2,000 molecules per cell [PubMed|21515776]

Biological materials

Mutant

• DS70 (''cheV''::''mls'' in NCIB3610) [Pubmed|12864845]
• BKE14010 ([gene|56E391BD4C154A603FFD06CEC7BC5615AAC5AD04|cheV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCAATCCCTCGCTATT, downstream forward: _UP4_TAAATAAAAACAGCCGTTGC
• BKK14010 ([gene|56E391BD4C154A603FFD06CEC7BC5615AAC5AD04|cheV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCAATCCCTCGCTATT, downstream forward: _UP4_TAAATAAAAACAGCCGTTGC

References

Reviews

• 18774298

Original publications

• 12864845,26122431,8169224,8169223,11553614,14731274,21098025,21515776,26844549