recN

DNA repair and genetic recombination

Locus: BSU_24240

Isoelectric point: 4.80

Molecular weight: 64.31 kDa

Protein length: 576 aa

Gene length: 1731 bp

Function: DNA repair and genetic recombination

Product: DNA integrity scanning protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins

Gene

Coordinates: 2,520,557 2,522,287

The protein

Catalyzed reaction/ biological activity

• RecN stimulates polymerizing activity of PnpA PubMed

Protein family

• recN family (single member, according to UniProt)

Structure

• 4AD8 (from Deinococcus radiodurans, 30% identity) PubMed

Localization

• nucleoid (multiple) PubMed
• evenly distributed in growing cells PubMed
• upon DNA damage, RecN forms a single focus per nucleoid PubMed, recruitment is affected by the presence of fumarase PubMed

Expression and Regulation

Operons

• Genes: ahrC-recN
  Description: PubMed

  Regulation

  • by sRNA sr1

  Additional information

  • expression is fourfold increased upon depletion of nusA Reference

• Genes: dxs-yqxC-ahrC-recN
  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

Biological materials

Mutant

• 1A891 ( recN::cat), PubMed, available at BGSC
• BKE24240 (recN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACAGCATTACACCTCTT, downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA
• BKK24240 (recN::kan trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_CAAACAGCATTACACCTCTT, downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA

Labs working on this gene/protein

• Peter Graumann, Freiburg University, Germany homepage

References

Reviews

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Original publications

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