asd

aspartate-semialdehyde dehydrogenase

locus: BSU_16750

pI: 4.97

mw: 37.69 kDa

protein length: 346 aa

gene length: 1041 bp

function: biosynthesis of threonine, lysine, dipicolic acid, peptidoglycan

product: aspartate-semialdehyde dehydrogenase

essential: yes

ec: 1.2.1.11

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

Gene

Coordinates: 1,745,991 1,747,031

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• L-aspartate 4-semialdehyde + NADP⁺ + phosphate --> 4-phospho-L-aspartate + H⁺ + NADPH (according to UniProt)

Protein family

• aspartate-semialdehyde dehydrogenase family (single member, according to UniProt)

Modification

• phosphorylation on Ser-98 AND Tyr-146 [Pubmed|17218307]

Structure

• [PDB|2GYY] (from ''Streptococcus pneumoniae'', 52% identity, 65% similarity) [Pubmed|16895909]

Expression and Regulation

Operons

• genes: [gene|564A2F43F8AABE97FC8F0B1B3382A9F31F02FA18|asd]-[gene|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG]-[gene|00B4CA4E25858A56F9D4E0ADEE2643F203FEC613|dapA]
  description: [Pubmed|8098035]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,8098035], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8098035], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

• genes: [gene|3DA80060554B78E9DC2189CECB78680095B08B32|spoVFA]-[gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]-[gene|564A2F43F8AABE97FC8F0B1B3382A9F31F02FA18|asd]-[gene|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG]-[gene|00B4CA4E25858A56F9D4E0ADEE2643F203FEC613|dapA]
  description: [Pubmed|8098035]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,8098035], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,8098035]

References

• 8345520,15699190,8098035,17218307