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noc [2020-11-11 10:08:51]

DNA-binding protein, spatial regulator of [SW|cell division] to protect the nucleoid, and timing device with an important role in the coordination of chromosome segregation and [SW|cell division], Z ring placement, coordination of early steps in DNA replication with establishment of a medial division site
locus
BSU_40990
pI
5.81
mw
32.64 kDa
protein length
283 aa Sequence Blast
gene length
852 bp Sequence Blast
function
control of [SW|cell division]
product
effector of nucleoid occlusion
essential
no
synonyms
yyaA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    4,207,897 4,208,748

    Phenotypes of a mutant

  • a ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]'' double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]

    • The protein

    Catalyzed reaction/ biological activity

  • binds specific sites on the chromosome [Pubmed|19494834]
  • prevents Z ring assembly over the bacterial nucleoid and helps fine tune the assembly of the Z ring at midcell during the cell cycle [pubmed|31152469]

    • Protein family

  • ParB family (with [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|ParB], according to UniProt)

    • Paralogous protein(s)

  • [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|ParB]

    • Structure

  • [PDB|1VZ0] ([protein|EF4BD49FCD49EE97908973581478951C8FA196C0|ParB] from Thermus thermophilus, 48% from aa 30 ... 219) [pubmed|15228524]

    • [SW|Localization]

  • associated to the cell membrane [Pubmed|25568309]

    • Expression and Regulation

    Operons

    genes
    [gene|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF]-[gene|7809B4F5B9172EBB6C91BDE7B673FB08E82763FF|gidA]-[gene|CCB7EE1D049585979303148FBDE0D42549E648DE|gidB]-[gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]
    description
    [Pubmed|11807071]

    regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|11948146], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: repression, [Pubmed|26020636], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]

    • regulation

  • constitutively expressed [Pubmed|23701187]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B874 (yyaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1873 NBRP B. subtilis, Japan]
  • BKE40990 ([gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACCTACACCACCTTT, downstream forward: _UP4_TAGAAGCTCTCCTGAAAAGC
  • BKK40990 ([gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACCTACACCACCTTT, downstream forward: _UP4_TAGAAGCTCTCCTGAAAAGC

    • GFP fusion

  • available in the [SW|Jeff Errington] lab

    • labs

  • Jeff Errington, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington Homepage]
  • David Rudner, Harvard, USA [http://rudnerlab.med.harvard.edu/ Homepage]

    • References

    Reviews

  • 19680248,19884039,25460802,26706151,28500529,31254421

    • Original Publications

  • 16549676,11807071,19494834,15210112,11948146,22457634,23701187,24097947,25568309,26020636,28723932,15228524,31152469