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yclN [2017-11-16 13:27:45]
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yclN [2017-11-16 13:27:45]

petrobactin (3.4-catecholate siderophore) ABC transporter (permease)
locus
BSU03800
pI
9.82
mw
34.75 kDa
protein length
316 aa Sequence Blast
gene length
948 bp Sequence Blast
function
acquisition of iron
product
petrobactin ABC transporter (permease)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    432,372 → 433,322

    The protein

    Catalyzed reaction/ biological activity

  • uptake of the siderophore petrobactin [Pubmed|19955416]

    • Protein family

  • FecCD subfamily (according to Swiss-Prot)

    • [SW|Localization]

  • cell membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]-[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]-[gene|473628F86C18FE9957841F3C8B45242C90CB341D|fpbP]-[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]
    description
    [Pubmed|19955416]

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
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    Biological materials

    Mutant

  • MGNA-C010 (yclN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2008 NBRP B. subtilis, Japan]
  • BKE03800 (Δ[gene|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTGCCTCCTTACATCC, downstream forward: _UP4_TTTATGCTGTTAAGGAGAAA
  • BKK03800 (Δ[gene|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTGCCTCCTTACATCC, downstream forward: _UP4_TTTATGCTGTTAAGGAGAAA

    • References

  • 10092453,19955416,12354229