narJ

chaperone for the nitrate reductase (protein J)

locus: BSU_37260

pI: 4.61

mw: 21.06 kDa

protein length: 184 aa

gene length: 555 bp

function: nitrate respiration, nitrogen assimilation

product: chaperone for the nitrate reductase (protein J)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.2|Anaerobic respiration]

Gene

Coordinates: 3,824,226 3,824,780

The protein

Protein family

• NarJ/NarW family (single member, according to UniProt)

Expression and Regulation

Operons

• genes: [gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
  description: [Pubmed|8846791,16428414]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: activation, [Pubmed|8846791,16428414], in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]

Biological materials

Mutant

• BKE37260 ([gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGTTACACCTCATTT, downstream forward: _UP4_AGCGTTGAAGGGGCTGTTCA
• BKK37260 ([gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTGTTACACCTCATTT, downstream forward: _UP4_AGCGTTGAAGGGGCTGTTCA

References

Reviews

• 11289299

Original publications

• 9352926,8846791,16428414