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glxA [2019-03-13 17:10:33]
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glxA [2019-03-13 17:10:33]

glyoxalase I
Locus
BSU38370
Isoelectric point
4.43
Molecular weight
14.29 kDa
Protein length
126 aa Sequence Blast
Gene length
381 bp Sequence Blast
Function
detoxification of methylglyoxal
Product
glyoxalase I
Essential
no
Synonyms
ipa-18r,ywbC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

    • Gene

    Coordinates
    3,937,135 3,937,515

    Phenotypes of a mutant

  • increased sensitivity to methylglyoxal PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • methylglyoxal + BSH - S-lactoyl-bacillithiol (BSH) PubMed

    • Protein family

  • glyoxalase I family (according to Swiss-Prot)

    • Structure

  • 1F9Z (from E. coli, 35% identity)

    • Expression and Regulation

    Operons

    Genes
    glxA
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-B221 (ywbC::erm), available at the NBRP B. subtilis, Japan
  • BKE38370 (glxA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTGCTCCTCCTCGTAA, downstream forward: _UP4_TAAAAATAAAGAACGTACAT
  • BKK38370 (glxA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTGCTCCTCCTCGTAA, downstream forward: _UP4_TAAAAATAAAGAACGTACAT

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • References

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