rbgA

assembly of the 50S subunit of the ribosome

Locus: BSU_16050

Isoelectric point: 9.53

Molecular weight: 31.83 kDa

Protein length: 282 aa

Gene length: 849 bp

Function: Ribosome assembly

Product: GTPase

Essential: yes

Synonyms: ylqF

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Ribosome assembly
• Information processing → Targets of second messengers → Targets of (p)ppGpp
• Groups of genes → Essential genes
• Groups of genes → GTP-binding proteins

Gene

Coordinates: 1,676,532 1,677,380

Phenotypes of a mutant

• essential PubMed
• depletion confers a slow growth phenotype, and induces an abnormality of the in vivo ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes PubMed
• premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including L16, L27, L28, L33, L35 and L36 PubMed

The protein

Catalyzed reaction/ biological activity

• proper positioning of L6 in the large subunit of the ribosome prior to incorporation of L16 PubMed
• Binds and hydrolyzes GTP and readily exchanges GDP for GTP
• stabilizes rRNA helices in the A and P functional sites PubMed

Protein family

• TRAFAC class YlqF/YawG GTPase family (together with CpgA and YqeH, according to UniProt)

Paralogous protein(s)

• ThdF, Obg, YqeH, YnbA, YphC, YyaF, Era, YsxC

Domains

• RNA-binding domain at the C-terminus PubMed
• CP-type G domain (aa 14-178) (according to UniProt)

Effectors of protein activity

• GTPase activity requires binding of L16 to the 50S subunit of the ribosome PubMed
• binds ppGpp PubMed, ppGpp inhibits activity PubMed

Structure

• 1PUJ
• 6PVK (complex of RbgA bound to ribosomal 50S subunit assembly intermediate) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: rbgA-rnhB-ylqG-ylqH
  Description: PubMed

Biological materials

Mutant

• MGNA-B141 (ylqF::erm), available at the NBRP B. subtilis, Japan

Labs working on this gene/protein

• Naotake Ogasawara, Nara, Japan

References

Reviews

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Original publications

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