opuAC

glycine betaine and arsenobetaine [SW|ABC transporter] (binding protein)

locus: BSU_03000

pI: 8.02

mw: 32.06 kDa

protein length: 293 aa

gene length: 882 bp

function: compatible solute transport

product: glycine betaine and arsenobetaine [SW|ABC transporter] (binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.4|Uptake of compatible solutes for osmoprotection]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 323,119 324,000

The protein

Catalyzed reaction/ biological activity

• binding for subsequent uptake of glycine betaine and arsenobetaine [pubmed|29159878,24561588]
• binding for subsequent uptake of dimethylglycine [Pubmed|24561588]

Structure

• [PDB|2B4L] (complex with glycine betaine)
• [PDB|3CHG] (in complex with DMSA)
• [PDB|5NXX] (in complex with arsenobetaine) [pubmed|29159878]

[SW|Localization]

• associated to the membrane (via [protein|5F989C57010ACFC03E37AF5A894153F432520921|OpuAB]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|FB2220730013A98D7C979062A4A451518B92DF09|opuAA]-[gene|5F989C57010ACFC03E37AF5A894153F432520921|opuAB]-[gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23175650], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]

  regulation

  • induced by osmotic stress [Pubmed|23175650]

Biological materials

Mutant

• BKE03000 ([gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT
• BKK03000 ([gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT

Expression vectors

• pGP2926 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

labs

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

Reviews

• 27935846

Original publications

• 10092453,9335265,16645306,16225868,16445940,7622480,18567662,21296969,22383849,23175650,23646920,24561588,25012968,29159878