ylaC

[SW|RNA polymerase] ECF-type [SW|sigma factor] YlaC

locus: BSU_14730

pI: 9.28

mw: 20.78 kDa

protein length: 173 aa

gene length: 522 bp

function: [category|SW 3.2.1|Transcription]

product: [SW|RNA polymerase] ECF-type [SW|sigma factor] YlaC

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]

Gene

Coordinates: 1,544,085 1,544,606

The protein

Protein family

• [SW|Sigma-70 factor family] (according to UniProt)
• [SW|ECF subfamily] (according to UniProt)

Effectors of protein activity

• [protein|B454D14B33E2C11F0BB0A1D1BB743FA64981A966|YlaD] acts as anti-sigma [Pubmed|16728958]

Structure

• [PDB|5WUQ] ([protein|search|SigW ]in complex with the cytoplasmic domain of [protein|4E720917045032E8B45CB8AF6E5C13AE0E48EE33|RsiW], 33% identity) [pubmed|28319136]

Expression and Regulation

Operons

• genes: [gene|6BB9336E8F5007274026A89BFC3744B52F28E42D|ylaA]-[gene|0703D458DB489F49150DD9E3477A87DC354A7B7F|ylaB]-[gene|544344B61A804F367BB726976E0C87B61998490A|ylaC]-[gene|B454D14B33E2C11F0BB0A1D1BB743FA64981A966|ylaD]
  description: [Pubmed|16501307]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16501307], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|544344B61A804F367BB726976E0C87B61998490A|YlaC]: sigma factor, [Pubmed|16501307], in [regulon|544344B61A804F367BB726976E0C87B61998490A|YlaC regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|16501307], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • [protein|search|Spx]: transcription activation [Pubmed|16501307]

• genes: [gene|544344B61A804F367BB726976E0C87B61998490A|ylaC]-[gene|B454D14B33E2C11F0BB0A1D1BB743FA64981A966|ylaD]
  description: [Pubmed|16501307]

  regulation

  • [protein|search|Spx]: transcription activation [Pubmed|16501307]

Biological materials

Mutant

• MGNA-B356 (ylaC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1355 NBRP B. subtilis, Japan]
• 1A908 ( ''ylaC''::''kan''), [Pubmed|15838020], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A908&Search=1A908 BGSC]
• BKE14730 ([gene|544344B61A804F367BB726976E0C87B61998490A|ylaC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCAATGGAATCCCTATGCT, downstream forward: _UP4_AAAAGTAGAGAGGAGGAGCG
• BKK14730 ([gene|544344B61A804F367BB726976E0C87B61998490A|ylaC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCAATGGAATCCCTATGCT, downstream forward: _UP4_AAAAGTAGAGAGGAGGAGCG

References

Reviews

• 24921931

Original publications

• 16501307,16728958,17675383,20817771,28319136,29760236