defB [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]

N-formylcysteine deformylase, required for the conversion of S-methyl-cysteine to cysteine

locus: BSU14560

pI: 5.00

mw: 20.00 kDa

function: utilization of S-methyl-cysteine

product: N-formylcysteine deformylase

essential: no

ec: 3.5.1.88

synonyms: ykrB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.1|Conversion of S-methyl cysteine to cysteine]

[SW|Categories] containing this gene/protein

• [SW|sulfur metabolism], [SW|sporulation/ other]

Gene

Coordinates on the chromosome (coding sequence): 1,526,195 -> 1,526,749

Phenotypes of a mutant

• no growth with S-methyl cysteine [Pubmed|23944997]
• inactivation of ''[SW|defB]'' reduces sporulation efficiency to 9% that of wild type cells; engulfment and fission defects, with reduced [SW|SigG] activity [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• N-acetylcysteine --> cysteine acetate [Pubmed|23944997]

Protein family

• polypeptide deformylase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|DefA]

Structure

• [PDB|1LQY] (complex with antibiotic actinonin, Geobacillus stearothermophilus)

Biological materials

Mutant

• MGNA-A901 (ykrB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/901 NBRP B. subtilis, Japan]

References

• 11429456,23944997,26735940