defB

N-formylcysteine deformylase, required for the conversion of S-methyl-cysteine to cysteine

Locus: BSU_14560

Isoelectric point: 5.46

Molecular weight: 20.51 kDa

Protein length: 184 aa

Gene length: 555 bp

Function: utilization of S-methyl-cysteine

Product: N-formylcysteine deformylase

Essential: no

E.C.: 3.5.1.88

Synonyms: ykrB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Sulfur metabolism → Conversion of S-methyl cysteine to cysteine

Gene

Coordinates: 1,526,195 1,526,749

Phenotypes of a mutant

• a defA defB double mutant is not viable unless the cells acquire suppressor mutations in fmt, glyA or folD PubMed
• no growth with S-methyl cysteine PubMed
• inactivation of defB reduces sporulation efficiency to 9% that of wild type cells; engulfment and fission defects, with reduced SigG activity PubMed

The protein

Catalyzed reaction/ biological activity

• N-acetylcysteine - cysteine + acetate PubMed
• H₂O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)

Protein family

• polypeptide deformylase family (with DefA, according to UniProt)

Paralogous protein(s)

• DefA

Structure

• 1LQY (complex with antibiotic actinonin, Geobacillus stearothermophilus, 67% identity) PubMed

Expression and Regulation

Operons

• Genes: defB
  Description: PubMed

Biological materials

Mutant

• MGNA-A901 (ykrB::erm), available at the NBRP B. subtilis, Japan
• BKE14560 (defB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA, downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC
• BKK14560 (defB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA, downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC

References

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