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pucJ [2019-06-06 11:26:26]
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pucJ [2019-06-06 11:26:26]

uric acid permease
Locus
BSU32430
Isoelectric point
9.64
Molecular weight
46.94 kDa
Protein length
449 aa Sequence Blast
Gene length
1350 bp Sequence Blast
Function
purine utilization
Product
uric acid permease
Essential
no
Synonyms
yunJ

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,330,502 3,331,851

The protein

Protein family

  • xanthine/uracil permease family (according to Swiss-Prot)
  • Paralogous protein(s)

    Structure

  • 3QE7 (E. coli uracil transporter, 31% identity) PubMed
  • Localization

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon
  • PucR: activation, PubMed, in PucR regulon
  • Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
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    Description

    Regulatory mechanism

  • PucR: auto-repression, PubMed, in PucR regulon
  • TnrA: activation, PubMed, in TnrA regulon
  • Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • view in new tab

    Biological materials

    Mutant

  • MGNA-A935 (yunJ::erm), available at the NBRP B. subtilis, Japan
  • BKE32430 (pucJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTATAGTCCCTCCCTGTA, downstream forward: _UP4_TTAGAAAAAGAGGTGTAAAA
  • BKK32430 (pucJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTATAGTCCCTCCCTGTA, downstream forward: _UP4_TTAGAAAAAGAGGTGTAAAA
  • References

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