ebrA [2019-07-10 12:03:42]

multidrug efflux transporter

locus: BSU17300

pI: 9.37

mw: 11.21 kDa

protein length: 105 aa

gene length: 318 bp

function: multidrug resistance

product: multidrug efflux transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.14|Multidrug exporters]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,865,058 1,865,375

The protein

Protein family

• paired small multidrug resistance protein family ([SW|PSMR family]) [Pubmed|17942072]
• [SW|drug/metabolite transporter (DMT) superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|74668FF19D097722D523CC54288B6409DE267631|EbrB]

Structure

• [PDB|6D0S] (human protein, 23% identity)

[SW|Localization]

• cell membrane [Pubmed|17417881]

Expression and Regulation

Operons

• genes: [gene|53576746FF1B644A1980F8ACD0E8F4A54CDDAD83|ebrA]-[gene|74668FF19D097722D523CC54288B6409DE267631|ebrB]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE17300 ([gene|53576746FF1B644A1980F8ACD0E8F4A54CDDAD83|ebrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATGAATTCTCCTCACT, downstream forward: _UP4_AATTGGCCGTAAAGGAGTGA
• BKK17300 ([gene|53576746FF1B644A1980F8ACD0E8F4A54CDDAD83|ebrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATGAATTCTCCTCACT, downstream forward: _UP4_AATTGGCCGTAAAGGAGTGA

References

Reviews

• 17942072

Original publications

• 17417881