gltT [2019-02-05 18:42:42]

major high-affinity Na⁺-coupled glutamate/ aspartate symport protein, uptakeof glyphosate

locus: BSU10220

pI: 9.03

mw: 45.76 kDa

protein length: 429 aa

gene length: 1290 bp

function: glutamate and aspartate uptake

product: major Na⁺-coupled glutamate/ aspartate symport protein

essential: no

synonyms: yhfG

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.1|Solute:sodium symporter family]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.6|Dicarboxylate/amino acid:cation symporter]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,096,560 1,097,849

Phenotypes of a mutant

• impaired uptake of glutamate and aspartate [Pubmed|25344233]

The protein

Catalyzed reaction/ biological activity

• uptake of glutamate and aspartate
• uptake of glyphosate and glufosinate [pubmed|30666812]

Protein family

• [SW|dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family] (according to UniProt)

Paralogous protein(s)

• [protein|622856EE642C42247C658DBD57E6A18C6E81FE58|GltP], [protein|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|DctP]

Kinetic information

• Km values: 37 5 M for glutamate, 41 9 M for aspartate [pubmed|25344233]

Effectors of protein activity

• aspartate uptake is competitively inhibited by glutamate [pubmed|29995990]

Structure

• [PDB|4IZM] (the protein from ''Pyrococcus horikoshii'', 35% identity, 73% similarity) [Pubmed|23563139]
• [http://pdb.org/pdb/explore/explore.do?structureId=4KY0 4KY0] (the glutamate transporter of ''Thermococcus kodakarensis'', 35% identity, 72% similarity) [Pubmed|24013209]

[SW|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BP233 (''[gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]''::''spc''), available in [SW|Fabian Commichau]'s lab
• BP235 (''[gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]''::''spc ''''[gene|622856EE642C42247C658DBD57E6A18C6E81FE58|gltP]''::''cat''), available in [SW|Fabian Commichau]'s lab
• GP2247 (''[gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]''::''ermC''), available in [SW|Jrg Stlke]'s lab
• GP2248 (''[gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]''::''aphA3''), available in [SW|Jrg Stlke]'s lab
• GP2722 (''[gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]''::''zeo''), available in [SW|Jrg Stlke]'s lab
• BKE10220 ([gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT
• BKK10220 ([gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT

Expression vectors

• for expression in ''B. subtilis'', in [SW|pAC7]: pBP56, available in [SW|Fabian Commichau]'s lab

References

• 18763711,23563139,22383849,24013209,25344233,14596613,10588697,29995990,30666812