ytnP

lactonase-homolog protein, inhibits the signalling pathway required for the streptomycin production and development of aerial mycelium in Streptomyces griseus

locus: BSU_29890

pI: 5.93

mw: 29.02 kDa

protein length: 256 aa

gene length: 771 bp

function: defense against competing bacteria

product: lactonase-homolog protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 3,058,631 3,059,401

The protein

Protein family

• [SW|metallo-beta-lactamase superfamily] (according to UniProt)

Modification

• phosphorylation on Ser-36 [Pubmed|17218307]

Structure

• [PDB|3ESH] (from Staphylococcus aureus, 50% identity)

Expression and Regulation

Operons

• genes: [gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]-[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A511 (ytnP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/511 NBRP B. subtilis, Japan]
• BKE29890 ([gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCGCCTCCGTCCATAT, downstream forward: _UP4_TAGCATATTTTTATGTTTAC
• BKK29890 ([gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCGCCTCCGTCCATAT, downstream forward: _UP4_TAGCATATTTTTATGTTTAC

References

• 17218307,22101040