sacX [2019-08-12 13:08:31]

sucrose permease of the [SW|phosphotransferase systems|phosphotransferase system], EIIBC of the [category|SW 1.2.2|PTS], [category|SW 3.4.3|Trigger enzyme], control of [protein|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY] activity

locus: BSU_38410

pI: 9.35

mw: 48.86 kDa

protein length: 459 aa

gene length: 1380 bp

function: sucrose uptake and phosphorylation, control of [protein|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY] activity

product: sucrose-specific [category|SW 1.2.2|PTS] permease, EIIBC component

essential: no

ec: 2.7.1.69

synonyms: ipa-14r,sacS

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]

Gene

Coordinates: 3,942,234 3,943,613

The protein

Catalyzed reaction/ biological activity

• Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

Protein family

• [category|SW 1.2.2|PTS] permease, sucrose family [Pubmed|10627040]

Paralogous protein(s)

• [protein|AE02C38397AA790E4B216BB6DBABFE907B984D05|SacP], [protein|BC568649A341B2E6341993EDA4BF52BDE18A3294|TreP], [protein|35E6C81BE481C3E57CF47B781580368C3AC15D83|BglP], [protein|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|MurP]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|531F132F7F6A878F1E1D56977B9898A14272349A|sacX]-[gene|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|sacY]
  description: [Pubmed|2116367]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1400159], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|1400159], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY]: antitermination, in [regulon|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY regulon]

  regulation

  • induction by sucrose (at high concentration) [Pubmed|8535520]

Biological materials

Mutant

• BKE38410 ([gene|531F132F7F6A878F1E1D56977B9898A14272349A|sacX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCAATTAAAACCTCCTT, downstream forward: _UP4_TAACTGGATTTATTCGATTT
• BKK38410 ([gene|531F132F7F6A878F1E1D56977B9898A14272349A|sacX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCAATTAAAACCTCCTT, downstream forward: _UP4_TAACTGGATTTATTCGATTT

References

• 10627040,2105292,2116367,1400159,8535520,30038046