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sacX
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sacX

sucrose permease of the phosphotransferase system, EIIBC of the PTS, Trigger enzyme, control of SacY activity
Locus
BSU_38410
Isoelectric point
9.35
Molecular weight
48.86 kDa
Protein length
459 aa Sequence Blast
Gene length
1380 bp Sequence Blast
Function
sucrose uptake and phosphorylation, control of SacY activity
Product
sucrose-specific PTS permease, EIIBC component
Essential
no
E.C.
2.7.1.69
Synonyms
ipa-14r,sacS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of sucrose
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of PRD-type regulators
  • Information processing → Regulation of gene expression → Trigger enzyme → Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a Cys residue

    • Gene

    Coordinates
    3,942,234 3,943,613

    The protein

    Protein family

  • PTS permease, sucrose family PubMed

    • Paralogous protein(s)

  • SacP, TreP, BglP, MurP

    • Domains

  • PTS EIIB domain type-1 (aa 1-86) (according to UniProt)
  • PTS EIIC domain type-1 (aa 106-459) (according to UniProt)

    • Localization

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    sacX-sacY
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • SacY: antitermination, in SacY regulon

    • Regulation

  • induction by sucrose (at high concentration) PubMed
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    Biological materials

    Mutant

  • BKE38410 (sacX::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGCAATTAAAACCTCCTT, downstream forward: _UP4_TAACTGGATTTATTCGATTT
  • BKK38410 (sacX::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGCAATTAAAACCTCCTT, downstream forward: _UP4_TAACTGGATTTATTCGATTT

    • References

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