metK [2019-02-24 18:03:06]

S-adenosylmethionine synthetase

locus: BSU30550

pI: 5.03

mw: 43.88 kDa

protein length: 400 aa

gene length: 1203 bp

function: S-adenosylmethionine synthesis

product: S-adenosylmethionine synthetase

essential: yes

ec: 2.5.1.6

synonyms: metE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,127,825 3,129,027

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + L-methionine + H₂O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)

Protein family

• AdoMet synthetase family (according to Swiss-Prot)

Structure

• [PDB|1FUG] (from ''Escherichia coli'', 63% identity, 75% similarity) [Pubmed|8723769]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|52D4DA0AA45F0774543786B55D18DF9DF53A5029|metK]
  description: [Pubmed|9387221]

  regulatory mechanism

  • [regulon|S-box|S-box]: transcription termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

  regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]

Biological materials

lacZ fusion

• pGP432 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab

References

• 10094622,16672621,9387221,18039762,15378759