metK [Mon May 16 2016 16:13:29 GMT+0200 (CEST)]

S-adenosylmethionine synthetase

locus: BSU30550

pI: 5.00

mw: 43.00 kDa

function: S-adenosylmethionine synthesis

product: S-adenosylmethionine synthetase

essential: yes

ec: 2.5.1.6

synonyms: metE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis/ acquisition of amino acids], [SW|essential genes], [SW|most abundant proteins]

This gene is a member of the following [SW|regulons]

• [SW|S-box]

Gene

Coordinates on the chromosome (coding sequence): 3,127,825 -> 3,129,027

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP L-methionine H₂O = phosphate diphosphate S-adenosyl-L-methionine (according to Swiss-Prot)

Protein family

• AdoMet synthetase family (according to Swiss-Prot)

Structure

• [PDB|1FUG] (from ''Escherichia coli'', 63% identity, 75% similarity) [Pubmed|8723769]

Expression and Regulation

Operon

• ''[protein|search|metK]'' [Pubmed|9387221]

Regulation

• induced by methionine starvation ([protein|search|S-box]) [Pubmed|10094622]

Regulatory mechanism

• [protein|search|S-box]: [SW|riboswitch], binds S-adenosylmethionine resulting in transcription termination [Pubmed|10094622]

Additional information

• belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

Biological materials

lacZ fusion

• pGP432 (in [protein|search|pAC7]), available in [SW|Stülke] lab

References

• 10094622,16672621,9387221,18039762,15378759