SubtiBank SubtiBank
metK
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metK

S-adenosylmethionine synthetase
locus
BSU_30550
pI
5.03
mw
43.88 kDa
protein length
400 aa Sequence Blast
gene length
1203 bp Sequence Blast
function
S-adenosylmethionine synthesis
product
S-adenosylmethionine synthetase
essential
yes
ec
2.5.1.6
synonyms
metE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    3,127,825 3,129,027

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O + L-methionine --> diphosphate + phosphate + S-adenosyl-L-methionine (according to UniProt)

    • Protein family

  • AdoMet synthase family (single member, according to UniProt)

    • Structure

  • [PDB|1FUG] (from ''Escherichia coli'', 63% identity, 75% similarity) [Pubmed|8723769]

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|52D4DA0AA45F0774543786B55D18DF9DF53A5029|metK]
    description
    [Pubmed|9387221]

    regulatory mechanism

  • [regulon|S-box|S-box]: transcription termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

    • regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
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    Biological materials

    lacZ fusion

  • pGP432 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

    • References

  • 10094622,16672621,9387221,18039762,15378759