metK

S-adenosylmethionine synthetase

Locus: BSU_30550

Isoelectric point: 5.03

Molecular weight: 43.88 kDa

Protein length: 400 aa

Gene length: 1203 bp

Function: S-adenosylmethionine synthesis

Product: S-adenosylmethionine synthetase

Essential: yes

E.C.: 2.5.1.6

Synonyms: metE

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of methionine/ S-adenosylmethionine
• Groups of genes → Essential genes

Gene

Coordinates: 3,127,825 3,129,027

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-methionine --> diphosphate + phosphate + S-adenosyl-L-methionine (according to UniProt)

Protein family

• AdoMet synthase family (single member, according to UniProt)

Structure

• 1FUG (from Escherichia coli, 63% identity, 75% similarity) PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: metK
  Description: PubMed

  Regulatory mechanism

  • S-box: transcription termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Regulation

  • induced by methionine starvation (S-box) PubMed

Biological materials

LacZ fusion

• pGP432 (in pAC7), available in Jörg Stülke's lab

References

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