SubtiBank SubtiBank
pucR
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pucR

transcriptional regulator of puc genes
Locus
BSU_32420
Isoelectric point
6.19
Molecular weight
60.34 kDa
Protein length
531 aa Sequence Blast
Gene length
1596 bp Sequence Blast
Function
regulation of purine utilization
Product
transcriptional regulator of puc genes (PucR family)
Essential
no
Synonyms
yunI

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,328,762 3,330,357

The protein

Protein family

  • PucR family
  • CdaR family (according to UniProt)
  • Additional information

  • information on binding sites can be found in the PRODORIC2 database
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • PucR: auto-repression, PubMed, in PucR regulon
  • TnrA: activation, PubMed, in TnrA regulon
  • Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
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    Biological materials

    Mutant

  • MGNA-A934 (yunI::erm), available at the NBRP B. subtilis, Japan
  • BKE32420 (pucR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGCGCATTCTCCTTTTC, downstream forward: _UP4_TAAAGTTTGTTACATTTTCT
  • BKK32420 (pucR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGCGCATTCTCCTTTTC, downstream forward: _UP4_TAAAGTTTGTTACATTTTCT
  • References

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