pucR

transcriptional regulator of puc genes

locus: BSU_32420

pI: 6.19

mw: 60.34 kDa

protein length: 531 aa

gene length: 1596 bp

function: regulation of purine utilization

product: transcriptional regulator of puc genes ([SW|PucR family])

essential: no

synonyms: yunI

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 3,328,762 3,330,357

The protein

Protein family

• [SW|PucR family]
• [SW|CdaR family] (according to UniProt)

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000058 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|52C1601482C26400A524E880334BB801F832D6ED|pucR]-[gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]-[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]-[gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]-[gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]
  description: [Pubmed|12823818]

  regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: auto-repression, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|25755103], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

  regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

Biological materials

Mutant

• MGNA-A934 (yunI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/934 NBRP B. subtilis, Japan]
• BKE32420 ([gene|52C1601482C26400A524E880334BB801F832D6ED|pucR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCGCATTCTCCTTTTC, downstream forward: _UP4_TAAAGTTTGTTACATTTTCT
• BKK32420 ([gene|52C1601482C26400A524E880334BB801F832D6ED|pucR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCGCATTCTCCTTTTC, downstream forward: _UP4_TAAAGTTTGTTACATTTTCT

References

• 12374841,11344136,12029039,12823818,25755103