yclK

two-component sensor kinase

locus: BSU_03760

pI: 6.15

mw: 54.88 kDa

protein length: 473 aa

gene length: 1422 bp

function: unknown

product: two-component sensor kinase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 427,247 428,668

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|706983E6942E883D3A9D45693E7B4015AEABE60B|YclJ]
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Paralogous protein(s)

• [protein|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|YvrG], [protein|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|YkoH], [protein|1582E81F7DA85F38D6732D341ABD0F052587F25A|KinE], [protein|4EE48E4931F662E51586DB6D01E91C688329222D|CssS]

[SW|Domains]

• two transmembrane segments
• [SW|HAMP domain] (aa 187-239) (according to UniProt)
• [SW|Histidine kinase domain] (aa 254-470) (according to UniProt)

Modification

• autophosphorylation on a His residue

Structure

• [PDB|4I5S] (from Streptococcus mutans, corresponds to the [SW|Histidine kinase domain], aa 240 ... 467, 33% identity) [pubmed|23468592]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]-[gene|52BB660B0CAB36C74F1D47963235846B45AF78AB|yclK]
  description: [Pubmed|15375128]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|15375128], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|15375128]

Biological materials

Mutant

• MGNA-C008 (yclK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2006 NBRP B. subtilis, Japan]
• BKE03760 ([gene|52BB660B0CAB36C74F1D47963235846B45AF78AB|yclK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTAGCAGCAGCTGATATA, downstream forward: _UP4_TAGCATACAGGGCGGCGCAT
• BKK03760 ([gene|52BB660B0CAB36C74F1D47963235846B45AF78AB|yclK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTTAGCAGCAGCTGATATA, downstream forward: _UP4_TAGCATACAGGGCGGCGCAT

References

• 10094672,15375128,23468592