epsO

similar to pyruvyltransferase, extracellular polysaccharide synthesis

Locus: BSU_34220

Isoelectric point: 9.63

Molecular weight: 37.12 kDa

Protein length: 322 aa

Gene length: 969 bp

Function: biofilm formation

Product: unknown

Essential: no

Synonyms: yvfF

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Matrix polysaccharide synthesis

Gene

Coordinates: 3,514,115 3,515,083

The protein

Protein family

• polysaccharide pyruvyl transferase family (with EpsI and YxaB, according to UniProt)

Structure

• 5AX7 (from yeast, corresponds to aa 79 ... 303, 30% identity) PubMed

Expression and Regulation

Operons

• Genes: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • RemA: activation, PubMed, in RemA regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in SinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

  Regulation

  • repressed by SinR PubMed

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed
  • expression is increased in rsiX mutants PubMed

Biological materials

Mutant

• MGNA-B608 (yvfF::erm), available at the NBRP B. subtilis, Japan
• BKE34220 (epsO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GGACAGTTTCTGTTTCAGGC, downstream forward: _UP4_CCTGCTCACATGTGAGCGGA
• BKK34220 (epsO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GGACAGTTTCTGTTTCAGGC, downstream forward: _UP4_CCTGCTCACATGTGAGCGGA

Labs working on this gene/protein

• Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Reviews

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Research papers

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The EAR RNA switch

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