trigger enzyme: mannitol-specific phosphotransferase system, EIICB of the PTS
function
mannitol uptake and phosphorylation, control of MtlR activity
product
trigger enzyme: mannitol-specific phosphotransferase system, EIICB
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins][category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.10|Utilization of mannitol][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue][SW|Categories] containing this gene/protein
[SW|phosphotransferase systems], [SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|trigger enzyme], [SW|membrane proteins], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|MtlR regulon]Gene
Coordinates on the chromosome (coding sequence)
449,724 -> 451,160
The protein
Catalyzed reaction/ biological activity
Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)Protein family
[SW|PTS] permease, fructose/ mannitol permease (Fru) family [Pubmed|10627040]Modification
phosphorylation on Ser-559 [Pubmed|17218307]Structure
[PDB|1A3A] (from ''Escherichia coli k12 mutant'', 42% identity, 66% similarity) [Pubmed|9551558][SW|Localization]
cell membrane (according to Swiss-Prot)[SW|Interactions]
[protein|search|MtlA]-[protein|search|MtlR] [Pubmed|20444094] (the non-phosphorylated IIB domain of [protein|search|MtlA] interacts with the two C-terminal EIIB (Gat)-like and EIIA (Mtl)-like domains of [protein|search|MtlR]) [Pubmed|23279188]Expression and Regulation
Operon
''[protein|search|mtlA]-[protein|search|mtlF]-[protein|search|mtlD]'' [Pubmed|12897001][SW|Sigma factor]
[protein|search|SigA] [Pubmed|22014119]Regulation
induced by mannitol ([protein|search|MtlR]) [Pubmed|20444094]repressed by glucose ([protein|search|MtlR]) [Pubmed|20444094]Regulatory mechanism
[protein|search|MtlR]: transcription activation [Pubmed|20444094]References
Reviews
Original publications
12897001,20444094,10627040,17218307,23279188,22014119 
