murAB [2019-08-12 11:33:31]

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

locus: BSU_37100

pI: 5.83

mw: 45.85 kDa

protein length: 429 aa

gene length: 1290 bp

function: peptidoglycan precursor biosynthesis

product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

essential: no

ec: 2.5.1.7

synonyms: murZ,lssF,rev-4

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]

Gene

Coordinates: 3,806,086 3,807,375

The protein

Catalyzed reaction/ biological activity

• Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)

Protein family

• EPSP synthase family (with [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|MurAA] and [protein|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|AroE], according to UniProt)

Paralogous protein(s)

• [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|MurAA]

Structure

• [PDB|3SG1] (from ''B. anthracis'', 50% identity, 81% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]
  description: [Pubmed|19270101]

  regulation

  • constitutive [Pubmed|19270101]

Biological materials

Mutant

• BKE37100 ([gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGTCTCCTTCCATA, downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG
• BKK37100 ([gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGTCTCCTTCCATA, downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG

Expression vectors

• pGP2595: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 21822642

Original publications

• 19270101