yaaN

unknown

Locus: BSU_00260

Isoelectric point: 5.60

Molecular weight: 43.67 kDa

Protein length: 386 aa

Gene length: 1161 bp

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

Gene

Coordinates: 36,478 37,638

The protein

Protein family

• TelA family (together with YceH) (according to UniProt)

Paralogous protein(s)

• YceH

Expression and Regulation

Operons

• Genes: xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
  Description: PubMed

• Genes: xpaC-yaaN-yaaO
  Description: PubMed

  Sigma factors

  • SigW: sigma factor, PubMed, in SigW regulon
  • SigK: sigma factor, PubMed, in SigK regulon

  Regulation

  • expressed in stationary phase

Biological materials

Mutant

• MGNA-B895 (yaaN::erm), available at the NBRP B. subtilis, Japan
• BKE00260 (yaaN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCATCGCTCTTACCCTCT, downstream forward: _UP4_TGATTACCAGTAAGTCTGGC
• BKK00260 (yaaN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCATCGCTCTTACCCTCT, downstream forward: _UP4_TGATTACCAGTAAGTCTGGC
• GP640 (yaaN::kan trpC2), available in Jörg Stülke's lab

Expression vector

• pGP2842: IPTG inducible expression, purification in E. coli with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab
• pGP2843: expression of Strep-yaaN by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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