SubtiBank SubtiBank
yfiY
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yfiY

[SW|ABC transporter for the siderophore schizokinen and arthrobactin (binding protein), works with ATPase YusV
Locus
BSU_08440
Isoelectric point
7.17
Molecular weight
36.15 kDa
Protein length
325 aa Sequence Blast
Gene length
978 bp Sequence Blast
Function
acquisition of iron
Product
ABC transporter for the siderophore schizokinen and arthrobactin (binding protein)
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → ABC transporters → Importers → Uptake of iron/ siderophores
  • Cellular processes → Homeostasis → Acquisition of iron → ABC transporters for the uptake of iron/ siderophores
  • Metabolism → Additional metabolic pathways → Iron metabolism → ABC transporters for the uptake of iron/ siderophores
  • Groups of genes → Secreted proteins
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

    • Gene

    Coordinates
    919,366 920,343

    Phenotypes of a mutant

  • poor growth with the xenosiderophores arthrobactin or aerobactin as single source of iron PubMed

    • The protein

    Protein family

  • Bacterial solute-binding protein 8 family (according to UniProt)

    • Domains

  • Fe/B12 periplasmic-binding domain (aa 56-325) (according to UniProt)

    • Modification

  • phosphorylation on (Ser-290 OR Thr-302) PubMed

    • Structure

  • 3TNY (from B. cereus, 57% identity)

    • Localization

  • associated to the membrane (via YfhA-YfiZ) PubMed
  • extracellular (signal peptide) PubMed

    • Expression and Regulation

    Operons

    Genes
    yfiY
    Description
    PubMed

    Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon

    • Regulation

  • repressed unless the cells enter an iron starvation (Fur) PubMed
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    Biological materials

    Mutant

  • MGNA-C311 (yfiY::erm), available at the NBRP B. subtilis, Japan
  • BKE08440 (yfiY::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTCCTCCCAATAT, downstream forward: _UP4_AAATAAAAAAAGACTCCGTC
  • BKK08440 (yfiY::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTCCTCCCAATAT, downstream forward: _UP4_AAATAAAAAAAGACTCCGTC

    • References

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