xlyA

N-acetylmuramoyl-L-alanine amidase, required for the formation of membrane vesicles in a sub-population of cells

Locus: BSU_12810

Isoelectric point: 5.34

Molecular weight: 31.76 kDa

Protein length: 297 aa

Gene length: 894 bp

Function: PBSX prophage-mediated lysis

Product: N-acetylmuramoyl-L-alanine amidase

Essential: no

E.C.: 3.5.1.28

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
• Prophages and mobile genetic elements → Prophages → PBSX prophage
• Groups of genes → Secreted proteins

Gene

Coordinates: 1,347,289 1,348,182

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• N-acetylmuramoyl-L-alanine amidase 2 family (according to UniProt)

Paralogous protein(s)

• XlyB, CwlA, CwlH

Domains

• contains a N-acetylglucosamine-polymer-binding LysM domain PubMed
• contains an amidase_2 domain (like BlyA, CwlA, CwlH, XlyB)
• N-acetylmuramoyl-L-alanine amidase domain (aa 45-140) (according to UniProt)
• LysM domain (aa 159-203) (according to UniProt)

Structure

• 3HMA PubMed

Localization

• extracellular (no signal peptide) PubMed

Expression and Regulation

Operons

• Genes: xhlA-xhlB-xlyA
  Description: PubMed

• Genes: xtmA-xtmB-xkdE-xkdF-xkdG-xkdH-xkdI-xkdJ-xkdK-xkdM-xkdN-xkzB-xkdO-xkdP-xkdQ-xkdR-xkdS-xkdT-xkdU-xkzA-xkdV-xkdW-xkdX-xepA-xhlA-xhlB-xlyA
  Description: PubMed

  Sigma factors

  • Xpf: sigma factor, PubMed, in Xpf regulon

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

Biological materials

Mutant

• BKE12810 (xlyA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCATCTCTCCTTATT, downstream forward: _UP4_TGATCAAAGACCATAAAAAT
• BKK12810 (xlyA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCATCTCTCCTTATT, downstream forward: _UP4_TGATCAAAGACCATAAAAAT

References

Reviews

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Original publications

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