SubtiBank SubtiBank
rppH [2019-09-03 11:59:32]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

rppH [2019-09-03 11:59:32]

decapping of NAD-capped RNAs, error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity
Locus
BSU_30630
Isoelectric point
5.95
Molecular weight
18.37 kDa
Protein length
158 aa Sequence Blast
Gene length
477 bp Sequence Blast
Function
DNA repair, RNA degradation
Product
8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase
Essential
no
Synonyms
mutTA,ytkD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Information processing → Genetics → DNA repair/ recombination
  • Information processing → Genetics → DNA repair/ recombination → Oxidized guanine (GO) DNA repair system
  • Information processing → RNA synthesis and degradation → RNases → RNA pyrophosphohydrolase
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

    • Gene

    Coordinates
    3,134,983 3,135,459

    The protein

    Catalyzed reaction/ biological activity

  • removal of pyrophosphate from the 5' end of mRNAs to make RNA accessible for degradation by RNases PubMed
  • RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position PubMed
  • decapping of NAD-capped RNAs PubMed
  • 8-oxo-dGTP + H2O --> 8-oxo-dGMP + diphosphate + H+ (according to UniProt)

    • Protein family

  • Nudix hydrolase (according to UniProt)

    • Structure

  • 4JZV PubMed

    • Expression and Regulation

    Operons

    Genes
    rppH
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigF: sigma factor, PubMed, in SigF regulon

    • Regulation

  • expressed during logarithmic growth (SigA) and early during sporulation in the forespore (SigF) PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A297 (ytkD::erm), available at the NBRP B. subtilis, Japan
  • BKE30630 (rppH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTATCCCTCAAATCC, downstream forward: _UP4_TAAAAAACCCGGCACATGTG
  • BKK30630 (rppH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTATCCCTCAAATCC, downstream forward: _UP4_TAAAAAACCCGGCACATGTG

    • References

    Reviews

    Loading

    Original publications

    Loading