mutTA [2017-8-11 16:0:3]

error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity

Locus: BSU30630

Isoelectric point: 5.95

Molecular weight: 18.37 kDa

Protein length: 158 aa

Gene length: 474 bp

Function: DNA repair, RNA degradation

Product: 8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase

Essential: no

Synonyms: ytkD

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Oxidized guanine (GO) DNA repair system
• Information processing → RNA synthesis and degradation → RNases → RNA pyrophosphohydrolase
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 3,134,983 → 3,135,459

The protein

Catalyzed reaction/ biological activity

• removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by RNases PubMed
• RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position PubMed

Protein family

• Nudix hydrolase family (according to Swiss-Prot)

Structure

• 4JZV PubMed

Expression and Regulation

Operons

• Genes: rppH
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigF: sigma factor, PubMed, in SigF regulon

  Regulation

  • expressed during logarithmic growth (SigA) and early during sporulation in the forespore (SigF) PubMed

Biological materials

Mutant

• MGNA-A297 (ytkD::erm), available at the NBRP B. subtilis, Japan

References

Reviews

Loading

Original publications

Loading