mutTA [2017-8-11 16:0:3]

error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity

locus: BSU30630

pI: 5.95

mw: 18.37 kDa

protein length: 158 aa

gene length: 474 bp

function: DNA repair, RNA degradation

product: 8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase

essential: no

synonyms: ytkD

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.5|Oxidized guanine (GO) DNA repair system]
• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.3|RNA pyrophosphohydrolase]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 3,134,983 → 3,135,459

The protein

Catalyzed reaction/ biological activity

• removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by [SW|RNases] [Pubmed|21925382]
• RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position [Pubmed|23610425,23610407]

Protein family

• [SW|Nudix hydrolase] family (according to Swiss-Prot)

Structure

• [PDB|4JZV] [Pubmed|23610407]

Expression and Regulation

Operons

• genes: [gene|50A50E33796EEF8046142EE928753C355EF42A1A|rppH]
  description: [Pubmed|9387221]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|14761999], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|14761999], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed during logarithmic growth ([protein|search|SigA]) and early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325]

Biological materials

Mutant

• MGNA-A297 (ytkD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/297 NBRP B. subtilis, Japan]

References

Reviews

• 22568516

Original publications

• 16513759,16497325,14761999,19011023,9387221,15576788,21925382,23610425,23610407