cymR

pleiotropic regulator of sulfur metabolism

locus: BSU_27520

pI: 9.88

mw: 11.75 kDa

protein length: 138 aa

gene length: 417 bp

function: regulation of sulfur metabolism

product: transcription repressor

essential: no

synonyms: yrzC

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.5|Regulators of core metabolism]

Gene

Coordinates: 2,811,717 2,812,133

Phenotypes of a mutant

• impaired growth in the presence of cystine and increased sensitivity to hydrogen peroxide-, disulfide-, paraquat- and tellurite-induced stresses [Pubmed|20608979]
• increased intracellular cysteine pool and hydrogen sulfide formation [Pubmed|20608979]
• depletion of branched-chain amino acids [Pubmed|20608979]

The protein

Protein family

• Rrf2 family of transcription regulators

[SW|Domains]

• [SW|HTH rrf2-type domain] (aa 2-125) (according to UniProt)

[SW|Cofactors]

• [protein|D1FC976597E5583E40A4ED7234FBCA743AB01354|CysK] acts as corepressor [Pubmed|18974048]

Structure

• [PDB|2Y75] [Pubmed|21624051]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000198 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
  description: [Pubmed|22383849]

• genes: [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]
  description: [Pubmed|16885442]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16885442], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Biological materials

Mutant

• MGNA-B515 (yrzC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1514 NBRP B. subtilis, Japan]
• BKE27520 ([gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATGAACACCTCTGTTA, downstream forward: _UP4_ATTTAGATCAGAGGTGTAAA
• BKK27520 ([gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACATGAACACCTCTGTTA, downstream forward: _UP4_ATTTAGATCAGAGGTGTAAA

labs

• [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

References

The [SW|CymR regulon]

• 16513748

Other original publications

• 18974048,17056751,16109943,16885442,11948165,15668000,20608979,21624051