yisQ

probable multidrug resistance protein

locus: BSU_10820

pI: 10.18

mw: 49.85 kDa

protein length: 455 aa

gene length: 1368 bp

function: unknown

product: putative multidrug exporter

essential: no

synonyms: yucE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.15|Multidrug exporters/ based on homology]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 1,160,776 1,162,143

The protein

Protein family

• [SW|MOP exporter family]
• [SW|multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family] (accordinig to UniProt)

Structure

• [PDB|3VVO] (MATE multidrug exporter from Pyrococcus furiosus, 23% identity) [pubmed|23535598]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|5050D0049B87C9FF2ACCE45BD13FDA0227AAB804|yisQ]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B182 (yisQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1181 NBRP B. subtilis, Japan]
• BKE10820 ([gene|5050D0049B87C9FF2ACCE45BD13FDA0227AAB804|yisQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGTCTAACCCCTTTC, downstream forward: _UP4_TAACACCCGGCTCAATCAAG
• BKK10820 ([gene|5050D0049B87C9FF2ACCE45BD13FDA0227AAB804|yisQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGTCTAACCCCTTTC, downstream forward: _UP4_TAACACCCGGCTCAATCAAG

References

Research papers

• 23535598