SubtiBank SubtiBank
yhfT [2018-01-30T22:07:26.000Z]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yhfT [2018-01-30T22:07:26.000Z]

similar to long-chain fatty-acid-CoA ligase, involved in surfactin production
locus
BSU10360
pI
5.95
mw
52.71 kDa
protein length
479 aa Sequence Blast
gene length
1437 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    1,110,479 → 1,111,918

    The protein

    Paralogous protein(s)

  • [protein|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|LcfA], [protein|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|LcfB], [protein|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|YngI]

    • Structure

  • [PDB|5BUQ] ([protein|16412BAB04BBF9FD1C3653328923687874C81868|MenE], 29% identitiy) [Pubmed|26276389]

    • Expression and Regulation

    Operons

    genes
    [gene|22215066A7BEA4E53189DE6639C19D1AD86DA7F4|yhfU]-[gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]-[gene|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|yhfS]-[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA]: repression, [Pubmed|11717296], in [regulon|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA regulon]

    • regulation

  • repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|11717296]

    • additional information

  • weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
    • view in new tab

    genes
    [gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]-[gene|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|yhfS]-[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]
    description
    [Pubmed|22383849]

    additional information

  • weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B280 (yhfT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1279 NBRP B. subtilis, Japan]
  • available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]
  • BKE10360 (Δ[gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTATGAGTAATTGTCATGT, downstream forward: _UP4_CTGGAAGAGAGTGTACAGTA
  • BKK10360 (Δ[gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTATGAGTAATTGTCATGT, downstream forward: _UP4_CTGGAAGAGAGTGTACAGTA

    • References

  • 20797616,22383849,12368242