serB

phosphoserine phosphatase, catalyzes the last step in serine biosynthesis

Locus: BSU_28940

Isoelectric point: 4.67

Molecular weight: 29.36 kDa

Protein length: 260 aa

Gene length: 783 bp

Function: biosynthesis of serine

Product: phosphoserine phoshatase

Essential: no

Synonyms: ysaA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of serine/ glycine/ alanine

Gene

Coordinates: 2,958,434 2,959,216

Phenotypes of a mutant

• auxotrophic for serine PubMed

The protein

Catalyzed reaction/ biological activity

• phosphoserine - serine + phosphate PubMed

Protein family

• HAD superfamily (according to UniProt)

Paralogous protein(s)

• YfnB

Structure

• 2GFH (from mouse, 32% identity)

Expression and Regulation

Operons

• Genes: serB
  Description: PubMed

Biological materials

Mutant

• MGNA-A998 serB::erm, available at the NBRP B. subtilis, Japan
• BKE28940 (serB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATCTTCTCCTCTGCTT, downstream forward: _UP4_TAAAAAAAGCATGATCTCTT
• BKK28940 (serB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATCTTCTCCTCTGCTT, downstream forward: _UP4_TAAAAAAAGCATGATCTCTT

References

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