ykkD [2019-06-07 10:11:29]

putative guanidine exporter

locus: BSU13100

pI: 8.62

mw: 11.19 kDa

protein length: 105 aa

gene length: 318 bp

function: export/ detoxification of guanidine

product: subunit of guanidine exporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.11|Other exporters]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,376,855 1,377,172

The protein

Protein family

• paired small multidrug resistance protein family ([SW|PSMR family]) [Pubmed|17942072]

Structure

• [PDB|3B5D] (from E. coli, 29% identity) [pubmed|18024586]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|5FD89F65E481CDC33B10A58C12C3168B929917ED|ykkC]-[gene|5001019AD87FEF7D7A920D570C1053D476F7F20C|ykkD]
  description: [Pubmed|10735877]

  regulatory mechanism

  • [protein|E9866855F0156859C45E98F3B385FE1263BB7EBC|YkkC riboswitch]: antitermination, [pubmed|28060483,27989440], in [regulon|E9866855F0156859C45E98F3B385FE1263BB7EBC|YkkC riboswitch regulon]

  regulation

  • induced in the presence of guanidine ([SW|ykkC riboswitch]) [Pubmed|27989440]

Biological materials

Mutant

• MGNA-A748 (ykkD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/748 NBRP B. subtilis, Japan]
• BKE13100 ([gene|5001019AD87FEF7D7A920D570C1053D476F7F20C|ykkD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAAACTGATCCAGTGCA, downstream forward: _UP4_TAAATTGATTTTTATCAAAT
• BKK13100 ([gene|5001019AD87FEF7D7A920D570C1053D476F7F20C|ykkD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAAACTGATCCAGTGCA, downstream forward: _UP4_TAAATTGATTTTTATCAAAT

References

Reviews

• 17942072

Original publications

• 10735877,21317561,27989440,27120414,18024586