ykkD [2018-10-10 18:41:12]

putative guanidine exporter

Locus: BSU13100

Isoelectric point: 8.62

Molecular weight: 11.19 kDa

Protein length: 105 aa

Gene length: 315 bp

Function: export/ detoxification of guanidine

Product: subunit of guanidine exporter

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Other exporters
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,376,855 → 1,377,172

The protein

Protein family

• paired small multidrug resistance protein family (PSMR family) PubMed

Structure

• 3B5D (29% identity)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ykkC-ykkD
  Description: PubMed

  Regulatory mechanism

  • YkkC riboswitch: antitermination, PubMed, in YkkC riboswitch regulon

  Regulation

  • induced in the presence of guanidine (ykkC riboswitch) PubMed

Biological materials

Mutant

• MGNA-A748 (ykkD::erm), available at the NBRP B. subtilis, Japan
• BKE13100 (ΔykkD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATAAACTGATCCAGTGCA, downstream forward: _UP4_TAAATTGATTTTTATCAAAT
• BKK13100 (ΔykkD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATAAACTGATCCAGTGCA, downstream forward: _UP4_TAAATTGATTTTTATCAAAT

References

Reviews

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Original publications

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