D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation
function
cell wall synthesis, cell elongation
product
endopeptidase-type autolysin
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.2|Autolytic activity required for peptidoglycan synthesis (cell elongation)][category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis][category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.4|Endopeptidases][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins][SW|Categories] containing this gene/protein
[SW|cell wall synthesis], [SW|cell wall degradation/ turnover]This gene is a member of the following [SW|regulons]
[SW|WalR regulon]Gene
Coordinates on the chromosome (coding sequence)
3,574,363 -> 3,575,784
Phenotypes of a mutant
a ''[protein|search|cwlO] [protein|search|lytE]'' mutant is not viable [Pubmed|17581128,22139507]shorter, fatter cells, this can be rescued by addition of Mg(2) [Pubmed|23869552,23855774] The protein
Catalyzed reaction/ biological activity
cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]Protein family
peptidase C40 family (according to Swiss-Prot)Paralogous protein(s)
the C-terminal D,L-endopeptidase domains of [protein|search|LytE], [protein|search|LytF], [protein|search|CwlS], and [protein|search|CwlO] exhibit strong sequence similarity[SW|Domains]
C-terminal D,L-endopeptidase domain [Pubmed|22139507]Effectors of protein activity
[protein|search|CwlO] requires activation by [protein|search|FtsE]-[protein|search|FtsX] [Pubmed|23869552,23855774]activity requires functional [protein|search|Mbl] [Pubmed|23869552][SW|Localization]
extracellular (signal peptide) [Pubmed|18957862]localizes to the outer lateral sidewall of the cell (via the N-terminal domain) [Pubmed|22139507]cell membrane in a [protein|search|FtsX]-dependent manner [Pubmed|23869552][SW|Interactions]
[protein|search|FtsX]-[protein|search|CwlO], at the membrane [Pubmed|23869552]Expression and Regulation
[SW|Sigma factor]
[protein|search|SigA] [Pubmed|24163346]Regulation
expressed during exponential growth ([protein|search|WalR]) [Pubmed|17581128]Regulatory mechanism
[protein|search|WalR]: transcription activation [Pubmed|17581128]Biological materials
Mutant
BKE34800 (''[protein|search|cwlO]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)References
Reviews
Original publications
21478646,16233686,17581128,20525796,18957862,20059685,22139507,23855774,23869552,24163346,27118079