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yrvO [2018-09-12 18:46:19]
cysteine desulfurase, sulfur transfer to TrmU, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules
function
tRNA modification
product
cysteine desulfurase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]Gene
Coordinates
2,810,559 → 2,811,698
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with [protein|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|TrmU]) [Pubmed|25825430]Protein family
[[Class-V pyridoxal-phosphate-dependent aminotransferase family]] (according to Swiss-Prot)Paralogous protein(s)
[protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|YcbU], [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|NifZ], [protein|88ACE6B79534338F7C72F107B35A0B2384007088|SufS], [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|NifS] Modification
phosphorylated on Arg-385 [Pubmed|22517742][SW|Cofactors]
PLPStructure
[PDB|1EG5] (from Thermotoga maritima, 37% identity, 54% similarity) [Pubmed|10715213]Expression and Regulation
Operons
genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
description
[Pubmed|22383849]
view in new tabgenes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]
description
[Pubmed|16885442]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16885442], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]view in new tabBiological materials
Mutant
MGNA-A828 (yrvO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/828 NBRP B. subtilis, Japan]References
16885442,11948165,10715213,22517742,12682299,17064282,25825430