maeA [2019-02-19 19:13:45]

malic enzyme, required for malate dependent catabolite repression of the [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB] operon

locus: BSU37050

pI: 4.88

mw: 63.93 kDa

protein length: 582 aa

gene length: 1749 bp

function: malate utilization

product: NAD-dependent malate dehydrogenase

essential: no

ec: 1.1.1.38

synonyms: ywkA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]

Gene

Coordinates: 3,800,418 3,802,166

The protein

Catalyzed reaction/ biological activity

• (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate - pyruvate

Protein family

• malic enzymes family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|BBBCD5F56779895189E21076AF165B901F654534|MalS]

[SW|Cofactors]

• NAD⁺

Expression and Regulation

Operons

• genes: [gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]-[gene|1E602BCB2A416CDBF2B5007765EC9628123FE979|ywkB]
  description: [Pubmed|12949160]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12949160], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR]: activation, [Pubmed|12949160], in [regulon|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|MalR regulon]

  regulation

  • expressed in the presence of malate ([protein|search|MalR]) [Pubmed|12949160]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B657 (ywkA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1656 NBRP B. subtilis, Japan]
• GP1141 (aphA3) available in [SW|Stlke] lab
• BKE37050 ([gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT
• BKK37050 ([gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT

labs

• [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

• 21815947,23136871,12949160,16788182,28974613