yubC [2019-08-09 08:28:39]

cysteine dioxygenase

locus: BSU_31140

pI: 5.05

mw: 17.98 kDa

protein length: 161 aa

gene length: 486 bp

function: unknown

product: cysteine dioxygenase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,193,863 3,194,348

The protein

Catalyzed reaction/ biological activity

• L-cysteine + O₂ = 3-sulfinoalanine (according to Swiss-Prot)

Protein family

• cysteine dioxygenase family (single membran, according to UniProt)

Structure

• [PDB|3EQE]
• [http://pdb.org/pdb/explore/explore.do?structureId=4qm8 4QM8] [Pubmed|25307852], [PDB|4QM9] (from Bacillus Subtilis 100% identity)

Expression and Regulation

Operons

• genes: [gene|4F296CA0094B8618224F998E1B382470EECEAB3C|yubC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A225 (yubC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/225 NBRP B. subtilis, Japan]
• BKE31140 ([gene|4F296CA0094B8618224F998E1B382470EECEAB3C|yubC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGTTTTTGCCTCCCTT, downstream forward: _UP4_TGATTGACAGATAAACTGCT
• BKK31140 ([gene|4F296CA0094B8618224F998E1B382470EECEAB3C|yubC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGTTTTTGCCTCCCTT, downstream forward: _UP4_TGATTGACAGATAAACTGCT

References

• 25307852,22383849