hmoB [2019-07-25 11:25:23]

heme monooxygenase

locus: BSU_10100

pI: 5.22

mw: 18.68 kDa

protein length: 166 aa

gene length: 501 bp

function: degradation of heme, acquisition of iron

product: heme monooxygenase

essential: no

synonyms: yixC,yhgC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]

Gene

Coordinates: 1,083,229 1,083,729

The protein

Catalyzed reaction/ biological activity

• binds hemin ''in vitro'' with ~1:1 stoichiometry and degrade hemin in the presence of an electron donor [Pubmed|21873409]

Protein family

• antibiotic biosynthesis monooxygenase family (with [protein|A4E1A1FA886980C863919DA011AC1EEDDBD74967|HmoA], according to UniProt)

Structure

• [PDB|3TVZ] [Pubmed|22531134]

Expression and Regulation

Operons

• genes: [gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]
  description: [Pubmed|8335642]

  regulation

  • constitutively expressed [Pubmed|21873409]

Biological materials

Mutant

• MGNA-B503 (yhgC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1502 NBRP B. subtilis, Japan]
• BKE10100 ([gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAGCCACCTCCTGCT, downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC
• BKK10100 ([gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAGCCACCTCCTGCT, downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC

References

• 8335642,22531134,24582752,21873409