ftsH [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

ATP-dependent metalloprotease

Locus: BSU00690

Isoelectric point: 5.00

Molecular weight: 70.00 kDa

Function: cell division, sporulation initiation

Product: ATP-dependent metalloprotease

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell division → Other genes
• Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Lifestyles → Coping with stress → Heat shock proteins
• Groups of genes → Membrane proteins

Categories containing this gene/protein

• cell division, proteolysis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), heat shock proteins, biofilm formation, membrane proteins, sporulation/ other

This gene is a member of the following regulons

• SigM regulon, TilS-HprT regulon

Gene

Coordinates on the chromosome (coding sequence): 76,984 -> 78,897

Phenotypes of a mutant

• defective in biofilm formation PubMed
• strongly reduced sporulation frequency PubMed, PubMed

The protein

Catalyzed reaction/ biological activity

• degrades Spo0E and Spo0M, resulting in reduced sporulation frequency in a ftsH mutant PubMed
• FtsH is involved in the degradation of EzrA PubMed
• degrades SpoIIE PubMed

Paralogous protein(s)

• YjoB

Effectors of protein activity

• protease activity is controlled by the interaction with the flottilins FloA and FloT PubMed

Structure

• 1LV7 (from E. coli, 64% identity) PubMed

Localization

• cell membrane PubMed
• preferentially at the division septum PubMed
• forms some foci in the membrane PubMed

Interactions

• forms hexamers PubMed
• SpoVM-FtsH PubMed
• FloT-FtsH PubMed
• FloA-FtsH PubMed
• FtsH-Spo0E PubMed
• FtsH-Spo0M PubMed
• SpoIIE-FtsH PubMed

Expression and Regulation

Operon

• ftsH PubMed
• tilS-hprT-ftsH PubMed

Sigma factor

• SigA PubMed, SigM PubMed

Regulation

• induced by heat shock (class III)

Regulatory mechanism

• TilS-HprT: transcription activation PubMed

Additional information

• the mRNA is very stable (half-life > 15 min) PubMed
• the FtsH levels are reduced in a 'floT floA' double mutant PubMed

Biological materials

Mutant

• 1A1061 ( ftsH::cat), PubMed, available at BGSC
• 1A1060 ( ftsH::erm), PubMed, available at BGSC

References

Reviews

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Original publications

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