SubtiBank SubtiBank
melC
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melC

ABC transporter for melibiose and raffinose (permease)
Locus
BSU_30290
Isoelectric point
9.85
Molecular weight
30.22 kDa
Protein length
276 aa Sequence Blast
Gene length
831 bp Sequence Blast
Function
uptake of melibiose and raffinose
Product
Essential
no
Synonyms
amyC

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,100,031 3,100,861

The protein

Protein family

Domains

  • ABC transmembrane type-1 domain (aa 69-261) (according to UniProt)
  • Structure

  • 4TQU (from Sphingomonas sp., 26% identity) PubMed
  • Localization

  • cell membrane PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • MelR: repression, PubMed, in MelR regulon
  • Regulation

  • induced by melibiose or raffinose (MelR) PubMed
  • repressed by glucose (CcpA) PubMed
  • there is an additional RNA "upshift" signal in front of the melE gene suggestive of a melE-melD-melC-melA mRNA PubMed. However, there is no promoter in front of melE, suggesting that this mRNA may be the product of mRNA processing PubMed
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    Biological materials

    Mutant

  • MGNA-A798 (amyC::erm), available at the NBRP B. subtilis, Japan
  • BKE30290 (melC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTTTTGTACGGGCGG, downstream forward: _UP4_GGTTAACATTAAAGGGGGAA
  • BKK30290 (melC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTTTTGTACGGGCGG, downstream forward: _UP4_GGTTAACATTAAAGGGGGAA
  • References

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