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gabT
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gabT

gamma-aminobutyrate transaminase, general stress protein
Locus
BSU_03900
Isoelectric point
5.30
Molecular weight
47.09 kDa
Protein length
436 aa Sequence Blast
Gene length
1308 bp Sequence Blast
Function
utilization of gamma-amino butyric acid
Product
gamma-aminobutyrate transaminase
Essential
no
E.C.
2.6.1.19
Synonyms
ycnG

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
441,571 → 442,881

Phenotypes of a mutant

  • growth defect in the presence of gamma-aminobutyrate PubMed
  • no growth with gamma-aminobutyrate as single carbon source PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • 4-aminobutanoate + 2-oxoglutarate --> succinate semialdehyde + L-glutamate (according to UniProt)
  • (S)-3-amino-2-methylpropanoate + 2-oxoglutarate --> 2-methyl-3-oxopropanoate + L-glutamate (according to UniProt)
  • the protein was reported to be involved in norspermidine production and biofilm disassembly PubMed; however, this is not the case PubMedand the original paper has been retracted
  • Protein family

    Paralogous protein(s)

    Cofactors

  • PLP (according to UniProt)
  • Structure

  • 1SF2 (from E. coli, 45% identity) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • GabR: activation, PubMed, in GabR regulon
  • Regulation

  • GabD: induced by stress (SigB) PubMed
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    Biological materials

    Mutant

  • MGNA-C068 (ycnG::erm), available at the NBRP B. subtilis, Japan
  • BKE03900 (ΔgabT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGAATATCCCCCTGTC, downstream forward: _UP4_TAATCATTGGAAAGAAAATG
  • BKK03900 (ΔgabT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGAATATCCCCCTGTC, downstream forward: _UP4_TAATCATTGGAAAGAAAATG
  • Labs working on this gene/protein

  • Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
  • References

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