gabT

gamma-aminobutyrate transaminase, general stress protein

Locus: BSU_03900

Isoelectric point: 5.30

Molecular weight: 47.09 kDa

Protein length: 436 aa

Gene length: 1308 bp

Function: utilization of gamma-amino butyric acid

Product: gamma-aminobutyrate transaminase

Essential: no

E.C.: 2.6.1.19

Synonyms: ycnG

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of gamma-amino butyric acid

Gene

Coordinates: 441,571 → 442,881

Phenotypes of a mutant

• growth defect in the presence of gamma-aminobutyrate PubMed
• no growth with gamma-aminobutyrate as single carbon source PubMed

The protein

Catalyzed reaction/ biological activity

• 4-aminobutanoate + 2-oxoglutarate --> succinate semialdehyde + L-glutamate (according to UniProt)
• (S)-3-amino-2-methylpropanoate + 2-oxoglutarate --> 2-methyl-3-oxopropanoate + L-glutamate (according to UniProt)
• the protein was reported to be involved in norspermidine production and biofilm disassembly PubMed; however, this is not the case PubMedand the original paper has been retracted

Protein family

• Class-III pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Paralogous protein(s)

• YhxA:

Cofactors

• PLP (according to UniProt)

Structure

• 1SF2 (from E. coli, 45% identity) PubMed

Expression and Regulation

Operons

• Genes: gabT-gabD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • GabR: activation, PubMed, in GabR regulon

  Regulation

  • GabD: induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-C068 (ycnG::erm), available at the NBRP B. subtilis, Japan
• BKE03900 (ΔgabT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGAATATCCCCCTGTC, downstream forward: _UP4_TAATCATTGGAAAGAAAATG
• BKK03900 (ΔgabT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGAATATCCCCCTGTC, downstream forward: _UP4_TAATCATTGGAAAGAAAATG

Labs working on this gene/protein

• Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

References

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