gamP [2017-8-11 16:0:3]

glucosamine-specific phosphotransferase system, EIICBA of the PTS

Locus: BSU02350

Isoelectric point: 5.47

Molecular weight: 67.97 kDa

Protein length: 631 aa

Gene length: 1893 bp

Function: glucosamine uptake and phosphorylation

Product: glucosamine-specific phosphotransferase system, EIICBA

Essential: no

E.C.: 2.7.1.69

Synonyms: ybfS, yzfA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Utilization of cell wall components
• Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of amino sugars
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of amino sugars
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a Cys residue
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue

Gene

Coordinates: 254,907 → 256,802

Phenotypes of a mutant

• delayed growth with glucosamine as the single carbon source PubMed

The protein

Catalyzed reaction/ biological activity

• protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

Protein family

• PtsI permease, glucose permease (Glc) family PubMed

Paralogous protein(s)

• NagP

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: gamA-gamP
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • GamR: repression, PubMed, in GamR regulon

  Regulation

  • induced by glucosamine (GamR) PubMed

Biological materials

Mutant

• MGNA-B937 (ybfS::erm), available at the NBRP B. subtilis, Japan
• QB6098 (aphA3), available in Stülke lab

References

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