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gamP [2018-07-26 14:55:57]
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gamP [2018-07-26 14:55:57]

glucosamine-specific permease of the phosphotransferase system, EIICBA of the PTS
Locus
BSU02350
Isoelectric point
5.47
Molecular weight
67.97 kDa
Protein length
631 aa Sequence Blast
Gene length
1893 bp Sequence Blast
Function
glucosamine uptake and phosphorylation
Product
glucosamine-specific PTS, EIICBA
Essential
no
E.C.
2.7.1.69
Synonyms
ybfS,yzfA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Utilization of cell wall components
  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of amino sugars
  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of amino sugars
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a Cys residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue

    • Gene

    Coordinates
    254,907 → 256,802

    Phenotypes of a mutant

  • delayed growth with glucosamine as the single carbon source PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

    • Protein family

  • PTS permease, glucose family PubMed

    • Paralogous protein(s)

  • NagP

    • Localization

  • membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    gamA-gamP
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • GamR: repression, PubMed, in GamR regulon

    • Regulation

  • induced by glucosamine (GamR) PubMed
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    Biological materials

    Mutant

  • MGNA-B937 (ybfS::erm), available at the NBRP B. subtilis, Japan
  • QB6098 (aphA3), available in Stülke lab
  • BKE02350 (ΔgamP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGACAGTCTCCTTTTATT, downstream forward: _UP4_TAAAAAAGTCCCCCCTGCTG
  • BKK02350 (ΔgamP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGACAGTCTCCTTTTATT, downstream forward: _UP4_TAAAAAAGTCCCCCCTGCTG

    • References

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