yycD

general stress protein, survival of ethanol stress

locus: BSU_40450

pI: 4.78

mw: 7.33 kDa

protein length: 66 aa

gene length: 201 bp

function: survival of ethanol stress

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 4,159,005 4,159,205

The protein

Structure

• [PDB|2ZF3] (from Chromobacterium violaceum, 31% identity)

Expression and Regulation

Operons

• genes: [gene|4DE499A579610BD576772AED84B1F4256A2418D6|yycD]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-B830 (yycD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1829 NBRP B. subtilis, Japan]
• BKE40450 ([gene|4DE499A579610BD576772AED84B1F4256A2418D6|yycD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCATTCCTCCCTTA, downstream forward: _UP4_TAATGAATGAAAGCCTTCGC
• BKK40450 ([gene|4DE499A579610BD576772AED84B1F4256A2418D6|yycD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCATTCCTCCCTTA, downstream forward: _UP4_TAATGAATGAAAGCCTTCGC

References

• 12823818,15805528,27766092